“Shoot regeneration from nodal segment of acacia Senegal in Borno of Nigeria” , Njidda M. Gadzama, Jummai T. Tarfa and Bukar H. Kabura
Njidda M. Gadzama, Jummai T. Kaldapa Martha Tarfa and Bukar H. Kabura Biotechnology Centre, University of Maiduguri P. M. B. 1069. Maiduguri, Nigeria email@example.com
Purpose: Acacia senegal is a valuable leguminous tree species of the Sudano/Sahelian region sought after for its economic and ecological importance. Developing in vitro propagation protocol for this tree in Borno State of Nigeria will provide a sustainable means of re-foresting and improving the nutrients of the degraded soil of the Sahel environment of Nigeria.
Design/Methodology/Approach: Nodal segments derived from 6-month old seedlings growing on the experimental farm of the Biotechnology Centre, University of Maiduguri, were cultured on Murashige and Skoog (MS) medium, supplemented with 0.025–1.5mg/l of 6-benzylaminopurine (BAP), alone and in combination with 0.02mg/l α-naphthalene acetic acid (NAA) and 0.2–1.6mg/l of kinetin (KN), alone or in combination with 0.2mg/l α-naphthalene acetic acid (NAA).
Findings/Results: The maximum number of shoots per explants (2.31 6 1.24) and longest shoots (2.59 6 1.38cm) were obtained from MS medium supplemented with 1.0mg/l and 1.5mg/l BAP respectively, while 1.16 6 0.71 shoots with maximum 2.34 6 1.35cm length were found in medium containing 1.2mg/l kinetin after four weeks of culture. Inclusion of NAA (0.02mg/1) with BAP at the various concentrations in the culture medium was not effective in enhancing shoot proliferation. However, obtained results indicate that both shoot number (1.70 6 0.95cm) and length (2.33 6 0.92cm) were enhanced by adding 0.2mg/l NAA to 0.4mg/KN.
Originality and value: This work would be the first attempt to propagate Acacia senegal by in-vitro method in Borno State of Nigeria, with the view of generating reproducible protocol for future mass propagation of the tree crop in the State.
Keywords: Acacia senegal; in vitro micro-propagation; gum arabic; induction; Murashige and Skoog (MS); Kinetin (KN); 6-Benzylaminopurine (BAP); α-naphthalene acetic acid (NAA); indole-3-acetic acid (IAA); indole-3-butyric acid (IBA)